| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GALINDO VEGA, Mauricio | FR | Colorado St. |
NT | |
| 2 | CAUBLE, Jack | FR | Colorado St. |
50.83 | raw:50.72 CSU Pueblo Spank Blasing/Open |
| 3 | DAHL, Tyler | JR | Wyoming |
50.23 | raw:50.12 Mountain West Indoor Track & Field Championships |
| 4 | COX, Jaymison | FR | Wyoming |
49.58 | raw:49.37 Championships at the Peak |
| 5 | WEETMAN, Elijah | FR | Colorado St. |
50.02 | raw:49.91 Doug Max Invitational |
| 6 | KNEESE, Troy | FR | Colorado Mines |
50.46 | raw:50.35 CSU Pueblo Spank Blasing/Open |
| 7 | THOMPSON, Drew | FR | Colorado St. |
53.77 | raw:53.66 CSU Pueblo Spank Blasing/Open |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAGUIRE, Joey | FR | Colorado Mines |
48.81 | raw:48.70 CSU-Pueblo Open/Early Bird |
| 2 | SMITH, Kendal | JR | Colorado |
47.75 | raw:47.64 Jack Christiansen Invitational |
| 3 | WILLIAMS, Tyler | FR | Colorado |
47.62 | 93rd Clyde Littlefield Texas Relays |
| 4 | SAYLES, Ronald | SR | Colorado |
47.23 | raw:47.12 Jerry Quiller Classic 2021 |
| 5 | MCCLURKIN, Duane | UNAT-Colorado | 47.60 | ||
| 6 | STARK, Adam | SO | Colorado St. |
47.64 | raw:47.53 Colorado Invitational 2019 |
| 7 | GILMORE, Ian | FR | Colorado |
48.09 | 93rd Clyde Littlefield Texas Relays |
| 8 | RAMOS, Josh | SR | Colorado |
49.50 |