| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Colorado |
B | Brooks | Christoper-Moody | Shults | Hill | DNS | |
| 3 | Northern Colorado |
B | Johnson | Johnson | Gregory | Turner | DNS | Tyson Invitational |
| 4 | Colorado |
A | Hill | Degenero | McArthur | Hurta | 3:45.00 | |
| 5 | Northern Colorado |
A | Moss | Schuetz | Petitt | Johnson | 3:48.16 | Tyson Invitational |
| 6 | Colorado Mines |
A | Higashi | Nelson | Moriarty | Wright | 3:52.45 | raw:3:52.011 CSU Pueblo Spank Blasing/Open |
| 7 | Wyoming |
A | Kearse | Renner | Burch | Davison | 4:05.00 |