| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wyoming |
B | Wetzel | Rodgers | Bruxvoort | Steiner | 3:27.00 | |
| 3 | Wyoming |
A | Cox | McComb | Dahl | Engebretsen | DNS | |
| 4 | Colorado |
A | Nelson | Monestime | McCoy | Hoy | DNS | 93rd Clyde Littlefield Texas Relays |
| 5 | Northern Colorado |
A | Soumah | Lyon | Hayward | Mootz | 3:11.04 | 2019 Triton Invitational |
| 6 | Colorado |
B | Ramos | Powell | Lindrud | Horton | 3:20.50 |