| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Northern Colorado |
A | Gregory | Lewis | Whitaker | Schuetz | 46.57 | raw:46.451 2019 Tom Benich Invite |
| 3 | Colorado St. |
B | Gonzalez | Hopkins | Rowe | Williams | DNS | |
| 4 | Colorado St. |
A | Bunson | Gale | Jasper | Ozoude | 44.50 | |
| 5 | Colorado |
A | Henderson | Welch | Badal | Glynn | 45.40 | 2019 Pac-12 Track & Field Championships |
| 6 | Northern Colorado |
B | Moss | Nesby | Johnson | Petitt | 46.57 | raw:46.451 2019 Tom Benich Invite |