| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Colorado |
A | Gilmore | Perry | Craig | Sayles | 41.06 | raw:40.941 Colorado Invitational 2019 |
| 4 | Northern Colorado |
A | Soumah | Lyon | N'Seboko | Cross | DNS | raw:40.461 Big Sky Outdoor Track & Field Championships |
| 5 | Colorado St. |
A | Eddings | Stephens | Fulton | Barnes | DNS | Mountain West Outdoor Track & Field Championship |