| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Jupiter Christian |
B | Gephart | Spence | Colman | Carrol | DNS |
| 2 | Abundant Life Christian |
A | Howard | Saint Fort | Roker | Ash | 58.50 |
| 3 | Jupiter Christian |
A | Winslow | Winslow | Fielder | Schiralli | 1:00.00 |
| 4 | Coral Springs Charter |
A | Leath | Dorvil | Pinkston | Sawyer | 52.01 |
| 5 | Westminster Academy |
A | Elmer | Murray | Freas | Lucas | 52.22 |
| 6 | Westminster Academy |
C | Reese | Jernigan | Heatherman | Carre | DNS |
| 7 | Westminster Academy |
B | Abreu | Mayorquin | Jones-Tynes | Hodel | 55.55 |
| 8 | Somerset Prep (NL) |
A | NT |