| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Westminster Academy |
B | Haymond | Tynes | Rodriguez | Scharer | NT |
| 2 | Jupiter Christian |
A | Fauntleroy | Brandon | WEDA | Evans | NT |
| 3 | Coral Springs Charter |
A | Lowe | Romano Jr. | Cordoba | Foster | 5:30.00 |
| 4 | Westminster Academy |
A | Nowicki | Gant | Sauer | Latimore | 3:56.00 |
| 5 | Olympic Heights |
A | Toribio | Ramsarran | Adrien | Machado Neto | 4:19.00 |
| 6 | Abundant Life Christian |
A | Wizzard | Joseph | Jackson | Wint Jr | NT |
| 7 | Somerset Prep (NL) |
A | Glass | Gipson | Kelly | Georges | NT |
| 8 | Somerset Prep (NL) |
B | Duffus | Guilloteau | Sanon | Garcia | NT |