| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Olympic Heights |
A | Yiadom | Makowski | Araujo | Pili | NT |
| 2 | Somerset Prep (NL) |
B | Maragh | Cunningham | Lubin | Duffus | 49.54 |
| 3 | Coral Springs Charter |
B | Clinkscale | Morgan | Blum | Mansel | 46.00 |
| 4 | Somerset Prep (NL) |
A | Guilloteau | Brown | Gipson | Glass | 43.68 |
| 5 | Abundant Life Christian |
A | Garnes | Wint Jr | Williams | Roker | 45.07 |
| 6 | Westminster Academy |
B | Tynes | Johnson | Paul | Blake | DNS |
| 7 | Westminster Academy |
C | Haymond | Lee | Bellis | Persand | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Westminster Academy |
A | Roch | Pierce | Gant | Gant | NT |
| 4 | Coral Springs Charter |
A | Yarbough | Marsh | Romano Jr. | Laroche | NT |
| 5 | Olympic Heights |
B | Louis | Ashiagbor | Filoramo | Lewis | NT |
| 6 | Jupiter Christian |
A | Zuniga | Fletcher | Fauntleroy | Mirabella | NT |