| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | A.D. Henderson | B | Porter | Henry | Cipyak | Gupta | NT |
| 2 | Coral Springs Charter |
B | Mikosz | Marinez | Louis | Ehrlich | 54.61 |
| 3 | A.D. Henderson | A | Robinson | Smith | Smith | O'Sullivan | 49.88 |
| 4 | St. Andrew's |
A | Whyte | Crage | Alise | Dyer | 44.07 |
| 5 | Coral Springs Charter |
A | Piering | Palomino | Zambrano | Donaldson | 45.50 |
| 6 | American Heritage Delray MS | A | Gold | Siddiq | Blanchard | Kendrick | 51.07 |
| 7 | St. Andrew's |
B | Hardin | Schmidt | Beaubrun | Domico | 55.95 |
| 8 | American Heritage Delray MS | B | Omer Kirimli | Deeg | Kyle | Calderon | NT |