| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BLOCK, Anna | 8 | Unattached | 2:15.79 | |
| 2 | STUART, Eloise | FR | Unattached | 2:15.89 | |
| 3 | AQUILINO, Kaycie | FR | St. Thomas (Fla.) |
2:24.00 | |
| 4 | WAITE, Andria | FR | Unattached | 2:25.00 | |
| 5 | TESTA, Lisa | Unattached | 2:30.00 | ||
| 5 | TESTA, Lisa | Unattached | 2:30.00 | ||
| 6 | MCKINNEY, Riley | FR | Southeastern U. |
2:30.79 | South Florida Invitational |
| 7 | GLOVER, Emani | FR | St. Thomas (Fla.) |
2:35.13 | STU Outdoor Meet |
| 8 | WHETRO, Amari | FR | St. Thomas (Fla.) |
2:37.97 | STU Under The Lights |
| 9 | CASTILLO, Valeria | FR | St. Thomas (Fla.) |
2:40.60 | STU Outdoor Meet |
| 10 | BETANCOURT, Mia | FR | Florida National | 2:42.64 | Nova Southeastern University Shark Invite |
| 11 | OKORO, Keelee | FR | Florida National | 2:42.64 | |
| 12 | PURNELL, Kortney | JR | Florida Memorial |
2:45.00 | |
| 13 | QUICHIMBO, Daryl | JR | Florida National | 2:55.98 | |
| 14 | RHETT, Amaurri | FR | St. Thomas (Fla.) |
NT |