| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLARKE, Malynte | FR | University of The Bahamas | 59.54 | |
| 2 | PIERRE-LOUIS, Wislande | SR | St. Thomas (Fla.) |
58.50 | UCF Black & Gold Challenge |
| 3 | RUBIO, Brianna | SR | Unattached | 57.13 | |
| 4 | HAYE-SMITH, Yanique | Unattached | 53.20 | ||
| 5 | BESS, Danielle | UNA | UNAT-St. Thomas (Fla.) | 55.20 | |
| 6 | SANDIFER, Ahlia | SR | St. Thomas (Fla.) |
58.34 | STU Under The Lights |
| 7 | ALLEN, Toniann | SR | Sheetah Athlete | 59.00 | |
| 8 | NEWBOLD, Marquell | FR | University of The Bahamas | 59.82 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HUTCHINSON, Isabelle | UNA | UNAT-St. Thomas (Fla.) | NT | |
| 4 | BULLARD, Michal | FR | University of The Bahamas | 1:00.00 | |
| 5 | RENE, Senyce | UNA | UNAT-Florida National | 1:07.17 |