| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAY, Bri | FR | St. Thomas (Fla.) |
24.78 | UCF Black & Gold Challenge |
| 2 | SANDIFER, Ahlia | SR | St. Thomas (Fla.) |
24.69 | 26.47 STU Open 3 |
| 3 | HORNES, Jasmin | SO | Warner |
24.20 | |
| 4 | MORRISON , Ebony | Unattached | 23.10 | ||
| 5 | HAYE-SMITH, Yanique | Unattached | 23.40 | ||
| 6 | DARRISAW, Khila | SO | St. Thomas (Fla.) |
24.50 | South Florida Invitational |
| 7 | WILSON, Dominique | SO | St. Thomas (Fla.) |
24.72 | UCF Black & Gold Challenge |
| 8 | PHILLIPS, Rhema | Rhema Phillips | 24.80 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CLARKE, Malynte | FR | University of The Bahamas | 25.66 | |
| 3 | RENE, Senyce | UNA | UNAT-Florida National | 26.99 | |
| 4 | JENKINS, Quanay'a | SO | Florida Memorial |
26.99 | 2025 Sun Conference Indoor Championship |
| 5 | ALLEN, Toniann | SR | Sheetah Athlete | 25.00 | |
| 6 | NEWBOLD, Marquell | FR | University of The Bahamas | 25.57 | |
| 7 | BURDON, Khy | FR | Florida National | 25.99 | 2025 Embry-Riddle Running Elements Classic College |
| 8 | BAKER, Aylannah | FR | Florida Memorial |
26.60 |