| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Husson |
A | Haughton | Kaniki | Scamman | Roberson | 44.70 |
| 3 | Southeastern U. |
A | Ayliffe | Laing | Official | Brown | 40.98 |
| 4 | Keiser |
A | Desouza | Goddard | Cruz | Le Fjeld | 40.85 |
| 5 | Campbellsville |
A | Campbell | Carr | Pouye | Baskerville | 40.95 |
| 6 | Defiance |
A | Barnes | Snell | Hall | Wilson | 41.85 |
| 7 | Florida National | A | Hall | Jimenez | Martinez | Agard | 44.18 |
| 8 | Ave Maria |
A | Burch | Enguita | Patterson | Kupcho | 44.74 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Campbellsville |
B | Johnson | Bain | Smith | Brice | 42.50 |
| 4 | Defiance |
B | Westmeyer | Selmon | Brown | Spikener | 45.52 |