| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Pompano Beach |
A | 43.68 | 2025 Coral Springs Invitational |
| 3 | Monarch |
A | 43.26 | Coconut Creek BCAA Regular Meet |
| 4 | Piper |
A | 42.63 | FHSAA 4A Region 4 |
| 5 | Piper |
B | DNS | FHSAA 4A Region 4 |
| 6 | Monarch |
B | 43.26 | Coconut Creek BCAA Regular Meet |
| 7 | Pompano Beach |
B | 45.00 |