| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Jupiter |
A | Martin | Baer | Havasi | Bellamy | 42.65 |
| 2 | Calvary Christian (FtL) |
A | Joseph | Charles | Ortiz | Cherry | 42.27 |
| 3 | Cardinal Gibbons |
A | Hadley Jr. | Stebbins | Findeson | Miller Jr | 41.40 |
| 4 | Miami Northwestern |
A | Fleming | Bain | Lawhorn | Briggs | 40.51 |
| 5 | NSU University |
A | Ambroise | Charles | Pupkin | Anderson | DNS |
| 6 | Archbishop McCarthy |
A | Alexandre | Denson | Blanc | Castro | 42.81 |
| 7 | Pine Crest |
A | Goin | Leon | Pritchard | Khouri | 42.62 |
| 8 | Pompano Beach |
A | Williams | Josaphat | Simon | Beharry | 43.25 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | AIE Charter |
A | Avila | Contreras | Garcia | Inoh | DNS |
| 2 | St. Andrew's |
A | Williams | St. Jean | Rahe | Boshell | DNS |
| 3 | Westminster Academy |
A | Pacienza | Fuentes | Ruck | Williams | 43.55 |
| 4 | Key West |
A | Dejean | Morin | Van Loon | Herron | 43.35 |
| 5 | Keys Gate |
A | Litzinger | Brooks | Bontemps | Howard | 43.61 |
| 6 | Somerset Prep (NL) |
A | Guilloteau | Brown | Gipson | Glass | 43.60 |
| 7 | Abundant Life Christian |
A | Larkin | Young | Jackson | Wint Jr | 45.07 |
| 8 | Divine Savior |
A | Blasi | Castillo | Oizan-Chapon | Gomez | 46.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Somerset Prep (NL) |
B | Swearing | Hall | Maragh | Ambroise | DNS |
| 2 | Archbishop McCarthy |
B | Grant | Vance | Jones | Antenor | 42.81 |
| 3 | South Florida HEAT |
B | Padgett | Poirier | Hsiao | Rodriguez | 1:05.44 |
| 4 | Mater Lakes |
A | Cruz | Jean | Brito | Viales | DNS |
| 5 | South Florida HEAT |
A | Long | Hathaway | Ablola | Ryle | 56.37 |
| 6 | Miami Northwestern |
B | Simmons | Wright | Louis | Wilson | 40.51 |
| 7 | Key West |
B | Gaertner | Oviedo | Guyet | Talpasz | DNS |
| 8 | Pine Crest |
B | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Pine Crest |
D | Pantoja | Burkowsky | Gibson | Haykov | 49.00 |
| 2 | Calvary Christian (FtL) |
C | Hunley | Pettiford | Adaka | Gil | DNS |
| 3 | AIE Charter |
B | Soler | Borjas | QUEIJA | Perez | NT |
| 4 | Calvary Christian (FtL) |
B | Henderson | Cherry | Piquant | Casimir | 42.27 |
| 5 | Pine Crest |
C | DNS | ||||
| 6 | AIE Charter |
C | Barrera-Leyva | Rosa | Velazco | Caballero | 47.47 |
| 7 | AIE Charter |
D | Arauz | Castro | Cote | Pichardo | DNF |