| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pine Crest |
A | Montague | Lang | Gutt | Green | 9:18.46 | 2019 FHSAA State Track & Field Championships |
| 2 | Benjamin School |
A | Lewis | Lemanski | Balistreri | Downey | 9:19.68 | FHSAA Class 1A Track & Field State Championships |
| 3 | Gulliver Prep |
A | Sawyer | Maury | Rodriguez | Malik | 9:48.83 | Sam Burley Hall of Fame Invitational |
| 4 | NSU University |
A | Nelson | Wickman | Starks | Goliakov | 10:31.78 | FHSAA 2A Region 4 |
| 5 | Oxbridge Academy |
A | Wolfe | Assad | Wajer | Platt | 11:00.71 | St Andrews Varsity |