| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Oxbridge Academy |
A | 4:35.00 | |
| 2 | John I. Leonard |
A | 4:19.30 | |
| 3 | Atlantic (Delray) |
A | 4:17.01 | 4:17.01 John I Leonard Invitational 2 |
| 4 | Pahokee |
A | 3:56.04 | FHSAA 1A Region 4 |
| 5 | Boynton Beach |
A | 4:13.66 | FHSAA 3A Region 4 |
| 6 | Palm Beach Gardens |
A | DNS | |
| 7 | Martin County |
A | 4:24.95 | 4:24.95 Falcon Classic 2024 |
| 8 | Suncoast |
A | 4:44.21 | 4:44.21 John I Leonard Invitational |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Seminole Ridge |
A | NT | |
| 3 | Glades Day |
A | DNS | |
| 4 | Somerset Canyons |
A | DNS | |
| 5 | Lake Worth |
A | 4:56.69 | 4:56.69 Boca Raton Invitational 2023 |
| 6 | Royal Palm Beach |
A | DNS | |
| 7 | West Boca Raton |
A | NT |