| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Somerset Canyons |
A | 50.85 | 50.85 John I Leonard Invitational 2 |
| 3 | West Boca Raton |
A | 50.00 | |
| 4 | Pahokee |
A | 49.42 | FHSAA 1A Region 4 |
| 5 | Palm Beach Gardens |
A | 49.68 | |
| 6 | Suncoast |
A | DNS | FHSAA 3A Region 4 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lake Worth |
A | NT | |
| 3 | Glades Day |
A | DNS | 54.35 Glade Central Invitational 2024 |
| 4 | Glades Central |
A | 52.59 | FHSAA 2A Region 4 |
| 5 | Martin County |
A | NT |