| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Monarch |
A | Deer | Fisher | Brady | Brannon | 43.12 |
| 2 | Atlantic (Delray) |
A | Horne | Horne | Davis | Timothee | DNS |
| 3 | Nova |
A | Carroll | Tilghman | Dunkley | Sparks | 41.77 |
| 4 | Dillard |
A | Jennings | West | Pinder | Smith | 40.83 |
| 5 | Flanagan |
A | Daniels | Thomas | Newby | Hawkins | 41.41 |
| 6 | Boyd Anderson |
A | Lamy | Stewart-McLaren | Tommie | Thomas | DNS |
| 7 | Douglas |
A | Gibbs | Davis | Burchell | Jackson | 42.70 |
| 8 | Coral Glades |
A | Raymonvil | Casseus | Wallace | Godfrey | 43.21 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Pompano Beach |
A | Josaphat | Beharry | Demarco | Jaouhar | 44.32 |
| 4 | Pembroke Pines Charter |
A | AKINBEHINJE | Patterson | Juste | BELL | DQ |
| 5 | Spanish River |
A | Toscano | Parrish | Frederick | Cheney | 43.71 |
| 6 | West Broward |
A | Williamson | Tonge | McPherson | Robinson | 44.92 |