| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | South Florida HEAT |
A | Diez | Diez | Chafton | Fee | 4:12.88 | FHSAA 1A Region 4 |
| 3 | Calvary Christian (FtL) |
A | Guerrier | Mitchell | Thomerson | Anthony | DNS | FHSAA 2A District 15 |
| 4 | Carrollton |
A | Lee | Hudson | Taracido | Rosso | 3:50.60 | FHSAA State Track & Field Championships (2A/Para-Athlete) |
| 5 | Boca Raton |
A | Taylor | Pimentel | Castillo | Uhl | 3:59.14 | 2019 FHSAA State Track & Field Championships |
| 6 | Coral Springs Charter |
A | Guerra | Hartley | Christian | Collymore | 4:10.43 | BCAA North Prelims |
| 7 | Carrollton |
B | Reinhard | Napoleon | Larrea | Nout | DNS |