| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jupiter Christian |
A | Fauntleroy | Shrank | Wilkerson | Baldwin | 45.38 | FHSAA 1A REGION 4 Track & Field Championship |
| 4 | Westminster Academy |
A | Cason | Pierce | Gant | Roch | 43.58 | FHSAA 1A Region 4 |
| 5 | Oxbridge Academy |
A | Ferguson | Quasha | Burrows | Patterson | 43.85 | P.V. Meet #1 |
| 6 | Westminster Academy |
B | Blake | Boeri | Albertson | Di Leo | 45.55 |