| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | American Heritage Jr. High | A | Cohen | Clark | Thomas | Parham | 56.00 |
| 2 | Coral Springs Charter |
A | Bastilla | Esteban | Guerra | Louis | 53.75 |
| 3 | South Florida HEAT | A | Heuglin | Ramos | Gargurevich | Callahan | 55.62 |
| 4 | Calvary Christian (FtL) |
A | Maurency | Stephenson | Linton | Moment | 47.11 |
| 5 | Somerset Prep (NL) |
A | Pierre | Brown | Bastien | Register | 52.27 |
| 6 | Atlantic Christian |
A | Haskins | Hughes | Walker | Chase | 52.49 |
| 7 | Abundant Life Christian Academ | A | Brown | Medelus | Sharpe | Saint Fort | 54.46 |
| 8 | King's Academy MS | A | Parrish | McClure | Moyeno | Williams | 56.61 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Abundant Life Christian Academ | B | Joseph | Less-Beezer | Joseph | Herbert | 54.46 |
| 2 | American Heritage Jr. High | B | Leighton | Paschal | Barsoum | Stauffer | 56.00 |
| 3 | Divine Savior |
A | Carrasquillo | Sarli | Peniche | Monsalve | 1:06.00 |
| 4 | Mater Bay Academy |
A | Colon | Arrazcaeta | Gonzalez | Wooley | 59.70 |
| 5 | Pine Crest Middle School | A | Harrington | Brocato | Wilson | Figueiredo | 58.00 |
| 6 | Parkridge Christian Academy | A | Winslow | Wallace | Demeglio | Brown | 59.13 |
| 7 | Calvary Christian (FtL) |
B | Brown | Sejour | Hunter | Zuniga | 47.11 |
| 8 | South Florida HEAT | B | Serrao | Leun | Alemany | Heuglin | 55.62 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Calvary Christian (FtL) |
D | McPherson | Charite | Lewis | Joseph | 47.11 |
| 2 | American Heritage Jr. High | C | Fields | Cherniawski | Thomas | Kelly | 56.00 |
| 3 | Pine Crest Middle School | B | Spitzig | Jiriyasin | Ganc | Jiriyasin | 1:05.00 |
| 4 | Calvary Christian (FtL) |
C | Dunn | Hartman | Rojas | Stoveall | DNS |
| 5 | King's Academy MS | B | Ruiz | Terga | O'Keefe | Rajawat | 56.61 |
| 6 | Coral Springs Charter |
B | Kauss | Garbizo | Pyne | Valdes | 1:01.49 |
| 7 | Pine Crest Middle School | C | Parsons-Carswell | Forrest | Leckey | Karnaby | 1:05.50 |