| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 548 | FABRA, Oscar | SR | Unattached | 58.14 |
| 2 | 163 | POLEDEROS, Stefanos | Alpha Omega Elite Track Team | 57.16 | |
| 3 | 551 | SUHAREVSKIS, Niks-Davis | SR | Unattached | 55.00 |
| 4 | 118 | FISHER, Sanjae | JR | Monarch | 54.04 |
| 5 | 562 | SANTACRUZ, Jeremiah | Hargrett Elite | 55.00 | |
| 6 | 124 | HENRY, Jayden | JR | Monarch | 55.25 |
| 7 | 173 | FRY, Harrison | Alpha Omega Elite Track Team | 57.89 | |
| 8 | 597 | LUCIEN, Jacques | Greater Philadelphia TC | 58.53 |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 3 | AZZOUNI, Yousef | 6 | Summit Questa Montessori | NT |
| 2 | 143 | MARCHESAN, Lucas | Alpha Omega Elite Track Team | 1:04.19 | |
| 3 | 557 | ARRIAGA-LISIER, Joseph | 8 | Unattached | 59.09 |
| 4 | 536 | ROONEY, Erik | Unattached | 58.90 | |
| 5 | 545 | HARRELL IV, Robert | 8 | Unattached | 59.03 |
| 6 | 132 | WORTHY, Isaiah | JR | Monarch | 1:00.09 |
| 7 | 24 | APOTHEKER, Brixton | 6 | Summit Questa Montessori | NT |
| 8 | 569 | HARGRETT, Chrisdon | Hargrett Elite | NT |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 576 | PIERRE, Gregory | Hargrett Elite | NT | |
| 2 | 572 | PIERRE, Giannis | Hargrett Elite | NT | |
| 3 | 574 | ST. PIERRE, Feguens | Hargrett Elite | NT | |
| 4 | 573 | SAINTIL JR, Kervens | Hargrett Elite | NT | |
| 5 | 567 | ST. PIERRE, Jack Jack | Hargrett Elite | NT | |
| 6 | 571 | JONES, Mason | Hargrett Elite | NT | |
| 7 | 564 | PIERRE, Giovani | Hargrett Elite | NT | |
| 8 | 568 | ST. PIERRE, Jackson | Hargrett Elite | NT |
| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 4 | 565 | HARGRETT II, Chrisdon | Hargrett Elite | NT |