| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | WILDGOOSE, Rachari | JR | Teon Elite | 57.75 |
| 2 | CHRISTIAN, Chelsea | Unattached | 58.23 | |
| 3 | NOEL, Olivia | Stride Stars | 1:02.00 | |
| 4 | ARNE, Raquel | Plantation H.E.A.T. | 1:04.12 | |
| 5 | RUIZ, Mariam | Miami Elite Track Club | 1:07.70 | |
| 6 | EDWARDS, Kaelyn | Plantation H.E.A.T. | 1:07.77 | |
| 7 | HERBERT, Axaiah | Stride Stars | 1:08.00 | |
| 8 | JEAN, Trinity | Stride Stars | 1:08.00 | |
| 9 | JACKSON, Jaesian | Stride Stars | 1:08.00 | |
| 10 | EBANKS, Raejanae | Stride Stars | 1:13.02 | |
| 11 | JOHNSON, Lois | Stride Stars | 1:14.00 | |
| 12 | MARTIAL, Jean'Nay | Stride Stars | 1:21.00 | |
| 13 | DAVIS, Jalilah | Plantation H.E.A.T. | NT |