| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | DUBUISSON, Jolie | JR | Unattached | 27.50 |
| 3 | CARTY-DUMITRESCU, Linda | Southwest Sprinters | 39.00 | |
| 4 | JOHNSON, Kayla | Professional Runners Club | 23.50 | |
| 5 | FRIEDLAND, Nicole | Unattached | 29.90 | |
| 6 | DUBUISSON, Tiana | FR | Unattached | 27.20 |
| 7 | HUGHES, Devyn | SO | Broward Elite | 29.00 |