| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SANDERS, Damyire | Greensboro Cheetahs | NT | |
| 2 | PLEASANT, Caymin | Lights Out Track and Field | NT | |
| 3 | GAVIN, Carter | Greensboro Blazers Track Club | NT | |
| 4 | FIELDS, Tre | Greensboro Bolts | NT | |
| 5 | FLETCHER, Jahir | Lights Out Track and Field | NT | |
| 6 | HARRISON, Oakland | Greensboro Cheetahs | NT | |
| 7 | ROSS, Jaxon | Lights Out Track and Field | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DURHAM, Kash | Unattached | NT | |
| 2 | HARVEY, Zamir | Lights Out Track and Field | 51.00 | |
| 3 | BRINSON, Zion | Tri-City Relays of NC | 43.00 | |
| 4 | RUSSELL JR, Adrian | Greensboro Blazers Track Club | 40.56 | |
| 5 | SMITH, Isaac | Tri-City Relays of NC | 42.00 | |
| 6 | KLUTTZ, Greyson Jean-Paul | Greensboro Blazers Track Club | 43.29 | |
| 7 | CLEMONS, Malachi | Second Gear Speed Track Club | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LEACH, Mason | Greensboro Blazers Track Club | 39.37 | |
| 2 | BODEN, Dallas | Tri-City Relays of NC | 39.00 | |
| 3 | WICKS, Kashton | Tri-City Relays of NC | 38.00 | |
| 4 | HARVEY, Dax | Lights Out Track and Field | 38.00 | |
| 5 | MCKINNEY, Keraun | Lights Out Track and Field | 38.00 | |
| 6 | HAYDEN, Zyion | Second Gear Speed Track Club | 38.70 | |
| 7 | TERRY, Rahji | Tri-City Relays of NC | 39.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GOWDY, Ahzir | Running Wild | 36.26 | |
| 2 | CHAVIS, Kason | Second Gear Speed Track Club | 34.20 | |
| 3 | WILLIAMS-GILFORD, Brennan | 40 & Elite | 32.31 | |
| 4 | MADISON, Quincy | Virginia Pride Track & Field C | 30.25 | |
| 5 | MADISON, Quincy | Unattached | 31.42 | |
| 6 | GILDYARD, Sire | Unattached | 33.84 | |
| 7 | BALDWIN, Jayden | Tri-City Relays of NC | 35.50 |