| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | JOHNSON, Isla | Greensboro Blazers Track Club | NT | |
| 3 | STOWE, Tiana | Second Gear Speed Track Club | NT | |
| 4 | SANDERS, Nallaya | Greensboro Cheetahs | NT | |
| 5 | POTTER, Ava | Lights Out Track and Field | NT | |
| 6 | SMITH, Kensley | Greensboro Cheetahs | NT | |
| 7 | SMITH, Dream | Solar Elite Athletics | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ALLSBROOK-ALLISON, Halo | Tri-City Relays of NC | 52.00 | |
| 2 | LEACH, Bailey | Greensboro Blazers Track Club | 48.26 | |
| 3 | MCRAE, Kylie | Lights Out Track and Field | 39.00 | |
| 4 | WILLIAMS-GILFORD, Ashby | 40 & Elite | 36.80 | |
| 5 | CHASE, Kamiyah | Honest Runners of North Miami | 37.00 | |
| 6 | TOMLIN, Alanna | Greensboro Blazers Track Club | 40.09 | |
| 7 | ALLSBROOK, Aurora | Tri-City Relays of NC | 50.00 | |
| 8 | MONTOUTH, Aliviyah | Greensboro Cheetahs | NT |