| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BREWINGTON, Karsyn | Unattached | NT | |
| 3 | HALL, Elise | Greensboro Blazers Track Club | 35.35 | |
| 4 | SCOTT, Elani | Greensboro Blazers Track Club | 33.57 | |
| 5 | AUTRY, Kaydence | Running Wild | 35.22 | |
| 6 | MCMILLIAN, Destinie | Tri-City Relays of NC | 41.50 | |
| 7 | NELSON-CRAWFORD, Amani | Tri-City Relays of NC | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DUMAS, Ta'Liyah | Greensboro Cheetahs | 32.46 | |
| 3 | CLEMONS, Maddison | Greensboro Blazers Track Club | 32.03 | |
| 4 | WALTERS, Nialani | Running Wild | 30.27 | |
| 5 | GOINS, Zoenova | Tri-City Relays of NC | 30.50 | |
| 6 | DAWKINS, Aubree | Steelers Track Club | 32.18 | |
| 7 | WILLIAMS, Meagan | Greensboro Blazers Track Club | 33.38 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HUGHES, Karma | Lights Out Track and Field | 29.03 | |
| 3 | THOMAS, Taylor | East End Lightning Youth | 28.50 | |
| 4 | SOLOMON, Lauren | East End Lightning Youth | 28.00 | |
| 5 | GRIFFIN, Maya | East End Lightning Youth | 28.50 | |
| 6 | BISHOP, Alyssa | Tri-City Relays of NC | 29.00 | |
| 7 | GEORGE, Laila | Running Wild | 29.14 |