| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DURHAM, Emory | Greensboro Blazers Track Club | NT | |
| 3 | MCCASKILL, Hassan | Tri-City Relays of NC | 32.00 | |
| 4 | DUBOSE, James | Lights Out Track and Field | 31.00 | |
| 5 | PORTER, Kameron | Greensboro Blazers Track Club | 31.80 | |
| 6 | JONES, Jeff | Greensboro Blazers Track Club | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DAVIS, Cameron | Greensboro Blazers Track Club | 28.21 | |
| 2 | WALKER JR, Tare | Tri-City Relays of NC | 26.00 | |
| 3 | KLUTTZ, Peyton | Greensboro Blazers Track Club | 25.51 | |
| 4 | KNIGHT, Najay | Greensboro Blazers Track Club | 23.93 | |
| 5 | SILLS, Lance | Greensboro Blazers Track Club | 25.17 | |
| 6 | MCDONALD, Zyon | Greensboro Cheetahs | 25.85 | |
| 7 | BOWLES, Jahari | Tri-City Relays of NC | 26.50 | |
| 8 | GEORGE, A'dris | Running Wild | 28.38 |