| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | ROBINSON, Skye | Greensboro Bolts | NT | |
| 4 | ANDERSON, Ky'Lee | Greensboro Cheetahs | NT | |
| 5 | BADGER, Trinty | Lights Out Track and Field | NT | |
| 6 | SERRANO, Charlotte | Greensboro Bolts | NT | |
| 7 | WARREN, Emerie | Greensboro Cheetahs | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HUGHES, Kali | Lights Out Track and Field | 50.00 | |
| 3 | HENLEY, Jordan | Steelers Track Club | 45.35 | |
| 4 | ROBINSON, Kyndall | Lights Out Track and Field | 44.00 | |
| 5 | CLEMONS, Makenzie | Greensboro Blazers Track Club | 44.25 | |
| 6 | PRINGLE, Laura | Steelers Track Club | 49.35 | |
| 7 | MONTOUTH, Lynnix | Greensboro Cheetahs | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GORY, Kalylah | Spartanburg Elite Track Club | 40.35 | |
| 2 | DAVIS, True | Stride For Purpose TC | 38.50 | |
| 3 | CURRY, Crislynn | Tri-City Relays of NC | 38.00 | |
| 4 | AUSTIN, Laiyah | East End Lightning Youth | 37.00 | |
| 5 | SOLOMON, Sade | East End Lightning Youth | 37.01 | |
| 6 | FITTS-SUTTON, Dior | Tri-City Relays of NC | 38.00 | |
| 7 | VANCE, Railyn | Tri-City Relays of NC | 40.00 | |
| 8 | TYLER, Emarie | Greensboro Blazers Track Club | 42.11 |