| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MORRIS, Kehlani | Greensboro Cheetahs | NT | |
| 4 | LYNCH, Bri'Asia | Greensboro Bolts | NT | |
| 5 | LITTLE, Sariya | Greensboro Cheetahs | NT | |
| 6 | LYNCH, Amari | Greensboro Bolts | NT | |
| 7 | HARRISON, Callee | Greensboro Cheetahs | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | SWEENEY, Victoria | Greensboro Cheetahs | NT | |
| 3 | PORTER, Kaydence | Greensboro Blazers Track Club | 36.85 | |
| 4 | WITHERSPOON, Denver | Greensboro Blazers Track Club | 35.00 | |
| 5 | PITTS, Carmen | Greensboro Blazers Track Club | 36.04 | |
| 6 | DAWKINS, Maddison | Steelers Track Club | 41.38 | |
| 7 | VARLACK, Yahmia | Greensboro Cheetahs | NT | |
| 8 | PAYTON, Anaysha | Greensboro Cheetahs | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JOHNSON, Iris | Greensboro Blazers Track Club | 33.58 | |
| 2 | MALONE, Ari | Tri-City Relays of NC | 33.00 | |
| 3 | WOFFORD, Kimora | Spartanburg Elite Track Club | 32.40 | |
| 4 | MATTHEWSON, Averee | Lights Out Track and Field | 32.00 | |
| 5 | MCMILLIAN, Dejah | Tri-City Relays of NC | 32.00 | |
| 6 | LONG, Kalani | Spartanburg Elite Track Club | 32.55 | |
| 7 | SOLOMON, Aaliyah | East End Lightning Youth | 33.00 | |
| 8 | TREJO, Tatianah | Greensboro Blazers Track Club | 34.80 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PAYLOR, Riley | Greensboro Blazers Track Club | 31.78 | |
| 2 | RICE, Tori | Primian Athletics | 30.52 | |
| 3 | CHERRY, A'nyla | Tri-City Relays of NC | 29.00 | |
| 4 | STOWE, Saleia | Second Gear Speed Track Club | 27.10 | |
| 5 | ANSLEY, Hailey | Second Gear Speed Track Club | 28.30 | |
| 6 | HARRIS, Journee | Isolele Trackstars | 30.47 | |
| 7 | WILLIAMSON, Brielle | Tri-City Relays of NC | 31.00 | |
| 8 | SILLS, Victoria | Greensboro Blazers Track Club | 31.83 |