| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Jasper | A | Brooks | Williams | LeBlanc | Waalee | 51.19 |
| 2 | Orange Little Cypress-Mauricevil | A | Le | Tippins | Meadows | Barclay | 48.87 |
| 3 | Vidor | A | Porterfield | Heater | Sanford | Roche | 51.55 |
| 4 | Bridge City | A | CARVER | Juneau | Augustine | LOBRADA | 52.55 |
| 5 | Silsbee | A | Norsworthy | Mitchell-Vaughn | Hafford | Hill | 47.99 |
| 6 | Lumberton High School | A | Borgobello | Reckart | Rodriguez | Ware | 52.41 |
| 7 | West Orange-Stark | A | JANICE | Wysong | Manuel | Miller | 51.30 |