| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | St. Edmund | A | Fontenot | Miller | Lavergne | Fontenot | NT |
| 2 | Erath | A | Mayard | Perro | Bouillion | Altamirano | 54.76 |
| 3 | Iota | A | Fontenot | James | Navarre | Spell | 51.44 |
| 4 | Crowley High School | A | Collins | Douresseaux | Patton | Conner | 53.76 |
| 5 | Basile | A | Fontenot | La Point | Martel | Ivory | 1:02.15 |