| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Dequincy | A | DarDar | Thompson | Seraille | Harrison | 5:33.48 |
| 3 | S. P. Arnett | A | Melton | Rousell | Lewis | Limon | 5:18.52 |
| 4 | Vinton | A | Boudreaux | Pankhurst | Dommert | Turner | 4:52.29 |
| 5 | Leblanc | A | Brown | Lavergne | Thibodeaux | Pearson | 4:59.90 |
| 6 | Ray D. Molo Magnet | A | Delafosse | Duhon | Humphrey | Scott | 5:25.71 |