| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Iowa | A | Cormier | Kerlegan | Charles | Pitre | 53.90 |
| 2 | Sam Houston | A | Buller | Louviere | Landry | Soileau | 53.82 |
| 3 | Sulphur | A | Landaverde | Trahan | Broussard | Judge | 53.50 |
| 4 | DeRidder | A | Miller | Taylor | Scott | Jackson | 51.68 |
| 5 | St. Louis | A | Williams | Jackson | Bergen | Pousson | 48.15 |
| 6 | LaGrange | A | Moss | Roy | Harbor | Ned | 53.14 |
| 7 | Washington-Marion Magnet | A | Coco | Tillman | Nealond | Chatman | 53.56 |