| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Oberlin | A | Manuel | Thomas | Rodriguez | Wolfe | NT |
| 2 | Kinder | A | Mitchell | Jones | Holliday | Harmon | NT |
| 3 | DeQuincy | A | Dardar | DeCloutte | Rogers | Marks | 54.53 |
| 4 | Grand Lake | A | Benton | Devall | McFarland | Leija | NT |
| 5 | Oakdale | A | Bradley | Jones | Young | Hardison | NT |
| 6 | South Beauregard | A | Gantt | Harrison | Crum | Scarlett | NT |