| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Eunice Junior | A | Semien | Pierre | Cormier | Edwards | NT |
| 2 | Hamilton Christian | A | Brown | Mouton | Butler | Williams | 1:02.18 |
| 3 | Iota | A | Fruge | James | Lopez | Navarre | 53.85 |
| 4 | Opelousas | A | LaFrance | Stephens | Shakesnider | Simien | NT |
| 5 | St. Edmund | A | Simon | McGee | Fontenot | Fontenot | 1:04.65 |
| 6 | Lawtell | A | Richard | Daniel | Guillory | McCartney | NT |