| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Anacoco | A | Provose | Dowden | Hyatt | Dowden | 1:05.02 |
| 3 | Deridder Junior | A | Robinson | Piper | Eugene | Green | 55.37 |
| 4 | South Beauregard | A | Marceaux | Brister | Jackson | Smith | 58.39 |
| 5 | Pitkin | A | Deason | Vidrine | Johnson | Wisby | NT |