| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Hoffman Estates (Conant) | A | Dugo | Danso | Jain | Whitaker | 1:41.50 |
| 3 | Buffalo Grove | A | Hopson | Alfaro | Prawica | Roser | DNS |
| 4 | Mt. Prospect (Prospect) | A | Beihoffer | Balenda | Raitano | Orlando | 1:28.90 |
| 5 | Winnetka (New Trier) | A | Vibhatasilpin | Anyaso | Pfister | Brewer | 1:34.89 |
| 6 | Glenview (Glenbrook South) | A | Freidinger | Collins | Hurst | Peters | 1:35.50 |
| 7 | Crystal Lake (Central) | A | O'Hare | Schroll | Stegmeyer | Runge | 1:42.24 |