| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Hoffman Estates (Conant) | A | Ariunbat | Corpin | Adenuga | Ralph | 4:02.00 |
| 3 | Winnetka (New Trier) | A | Boeing | Landwer | Hayes | Salles | 3:50.28 |
| 4 | Mt. Prospect (Prospect) | A | LoBue | Numani | Connolly | Knee | 3:45.00 |
| 5 | Glenview (Glenbrook South) | A | Clough | Kogut | Fontana | Qualkenbush | 3:48.00 |
| 6 | Crystal Lake (Central) | A | Fujino | Hopkins | Richmond | McIntyre | 3:52.24 |
| 7 | Belvidere (North) | A | Kramer | Schroeder | Ostalaza | Beutel | 4:08.00 |