| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Glenview (Glenbrook South) | A | Spilios | Clough | Cotton | Zambianchi | 46.80 |
| 4 | Winnetka (New Trier) | A | Hirschle | Bloom | Corboy | Anglemyer | DQ |
| 5 | Mt. Prospect (Prospect) | A | Easter | Chavez | Deleon | Orlando | 45.80 |
| 6 | Buffalo Grove | A | Bey | Stephens | Carey-Marshall | Chen | 49.50 |
| 7 | Hoffman Estates (Conant) | A | Burke | Ralph | Arceo | Halupka | DQ |