| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | WILLIAMS, Jabari |  Florida A&M | 49.50 | UCF Black & Gold Invite | 
| 2 | SMITH, Jordan |  Albany State | 48.44 | SIAC Championships | 
| 3 | KELLY, Jonathan |  Albany State | 48.22 | 2024 Morehouse Relays | 
| 4 | SELDERS, Cameron |  Morehouse | 47.43 | |
| 5 | SIMMONS JR., Michael |  Morehouse | 47.61 | |
| 6 | TERRY, David |  Paine | 48.40 | 2024 Morehouse Relays | 
| 7 | MORRIS, Sidney |  Morehouse | 49.43 | |
| 8 | MAPSON, Tyler |  Albany State | 49.50 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | HEARD, Isiah |  Morehouse | 51.00 | |
| 2 | HARMON, Kade |  Columbus St. | 50.56 | UWG Legends Track Meet | 
| 3 | JACKSON, Demario |  Albany State | 50.00 | |
| 4 | VESSAT, Samuel |  Edward Waters | 49.72 | South Florida Invitational | 
| 5 | GIDDENS, Jeremiah |  West Georgia | 50.00 | |
| 6 | BAKER, Dawson |  Florida A&M | 50.28 | Embry-Riddle Running Elements Classic - Collegiate and Open | 
| 7 | RUDOLPH, Jaden |  Fort Valley State | 51.00 | Don McGarey Invitational | 
| 8 | GARCIA, Anthony |  West Georgia | 51.00 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BROWN JR., Anthony |  Columbus St. | 52.66 | UWG Legends Track Meet | 
| 2 | KWADEY, Christian | Unattached | 52.50 | |
| 3 | EDWARDS, Malik |  Flagler | 51.63 | Embry-Riddle Running Elements Classic - Collegiate and Open | 
| 4 | FEEZOR, Tim |  West Georgia | 51.00 | |
| 5 | MCCANTS, Jaquan |  Edward Waters | 51.29 | North Florida Collegiate Invitational | 
| 6 | MCGRORTY, Ian |  Flagler | 51.92 | The Spartan Classic | 
| 7 | FLORES, Elijah |  Morehouse | 52.62 | Emory Thrills in the Hills 2024 | 
| 8 | HARVEY, Terrance | Indiana | 52.89 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | JENNINGS, Connor |  Albany State | 54.57 | Emory Spring Break Classic | 
| 3 | EBERHART, TaQuarus |  Columbus St. | 53.88 | UWG Legends Track Meet | 
| 4 | BROWN, Keashawn | Unattached | 53.00 | |
| 5 | BRYANT, Taquarrion |  Fort Valley State | 53.44 | Bob Hayes Spring Break Invitational - Collegiate | 
| 6 | BROOKS, Kenneth |  Columbus St. | 54.13 | UWG Legends Track Meet |