| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | MONTEFUSCO, Kira |  West Georgia | 25.62 | UWG Legends Track Meet | 
| 2 | SIMMONS, Kennedy |  Albany State | 25.50 | |
| 3 | KONE, Emmanuella |  Edward Waters | 24.74 | 2022 SAC Outdoor Track & Field Championship | 
| 4 | BERRY, Shaniya |  Florida A&M | 24.35 | The Sun Conference Outdoor Championships | 
| 5 | AUSTELL, D'liyah |  Fort Valley State | 24.61 | UWG Legends Track Meet | 
| 6 | GILYARD, Dasia |  Albany State | 25.36 | 2022 SIAC Championships | 
| 7 | LAWRENCE, Gabriella |  Paine | 25.55 | |
| 8 | MINTER, Dasani |  West Georgia | 25.67 | UWG Legends Track Meet | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CLARKE, Bridgette |  Fort Valley State | 26.84 | 2024 Morehouse Relays | 
| 2 | BROWN, Campbell |  West Georgia | 26.47 | Gamecock Opener 2023 | 
| 3 | JONES, Imani |  Albany State | 26.00 | |
| 4 | CAGE, Calyanna |  Fort Valley State | 25.70 | |
| 5 | LAWRENCE, Jeniya |  Albany State | 25.78 | Emory Spring Break Classic | 
| 6 | MONTGOMERY, Nakhia |  Columbus St. | 26.15 | UWG Legends Track Meet | 
| 7 | CRIMES, Chavondria |  Albany State | 26.81 | 2024 Morehouse Relays | 
| 8 | PERRY, Shamiya |  West Georgia | 27.00 | Gulf South Indoor Track & Field Championship | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BROWN, Valerie |  Edward Waters | 28.05 | 2024 Morehouse Relays | 
| 2 | MOORE, Markayla |  Columbus St. | 27.92 | UWG Legends Track Meet | 
| 3 | ZINNIE, Zoe |  Flagler | 27.64 | Bob Hayes Spring Break Invitational - Collegiate | 
| 4 | STONE, Termetrius |  Albany State | 27.22 | Emory Spring Break Classic | 
| 5 | DOWNER, Deandra |  Columbus St. | 27.25 | Emory Spring Break Classic | 
| 6 | KLECKLEY, Kaleah |  Albany State | 27.91 | 2024 Morehouse Relays | 
| 7 | THOMAS, Maniyah |  West Georgia | 28.04 | BSC Panther Indoor Icebreaker | 
| 8 | TATE, Jerrianna |  Albany State | 28.19 | Georgia Southern Spring Classic | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BYRD, Destiny |  Edward Waters | 30.12 | South Florida Invitational | 
| 2 | ONEAL, Bailey |  Albany State | 29.48 | Embry Riddle Blue and Gold Meet | 
| 3 | KERN, Julianna |  Albany State | 29.00 | |
| 4 | ELDER, Amber |  Columbus St. | 28.51 | Emory Thrills in the Hills Open | 
| 5 | PINNOCK, Shania |  Columbus St. | 28.91 | Emory Spring Break Classic | 
| 6 | HARLAN, Denyla |  Edward Waters | 29.28 | Bob Hayes Spring Break Invitational - Collegiate | 
| 7 | LACY, Syni |  Columbus St. | 30.06 | Clark Atlanta University Panther Track and Field Classic | 
| 8 | MELENDEZ ARCE, Annmary | Wesleyan (Ga.) | 30.53 | Collegiate Conference of the South Championships | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | BAILEY, Tyloni |  Albany State | NT | |
| 3 | DOLL, Alexis |  West Georgia | NT | |
| 4 | MONTGOMERY, Narissa | Wesleyan (Ga.) | 32.10 | Don McGarey Invitational | 
| 5 | RIDLEY, Jaeva |  West Georgia | NT | |
| 6 | HAMPTON, Taylor |  West Georgia | NT |