| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | KWADEY, Christian | Unattached | 21.50 | |
| 2 | BELTON, Kyron |  Thomas (Ga.) | 21.44 | UWG Legends Track Meet | 
| 3 | BROWN, Brian |  Paine | 21.11 | |
| 4 | JENKINS, Da'Veon |  Fort Valley State | 22.09 | Bob Hayes Spring Break Invitational - Collegiate | 
| 5 | GILBERT, Ed |  Albany State | 20.97 | 2022 SIAC Championships | 
| 6 | DALTON, Jailen |  Columbus St. | 21.23 | 2023 Peach Belt Conference Outdoor Track Championship | 
| 7 | BURDEN, Tahjay |  Paine | 21.44 | |
| 8 | WILLIAMS, Javian | Most Valuable Athletics | 21.50 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | GIBBS, Kody |  Edward Waters | 22.16 | UCF Knights Invite | 
| 2 | MOORE, Kibren |  Albany State | 20.78 | Emory Spring Break Classic | 
| 3 | NELSON, Jermaine |  Thomas (Ga.) | 21.98 | FSU Relays | 
| 4 | COOKS II, Cornelius |  Fort Valley State | 21.73 | Bob Hayes Spring Break Invitational - Collegiate | 
| 5 | WILLIAMS, Weylon |  Fort Valley State | 21.94 | 2024 Morehouse Relays | 
| 6 | REID, Isaiah |  Edward Waters | 22.04 | South Florida Invitational | 
| 7 | HUTCHINSON, Jayvon |  Albany State | 22.13 | Running Eagles Open | 
| 8 | GIDDENS, Jeremiah |  West Georgia | 22.18 | UWG Legends Track Meet | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BAKER, Dawson |  Florida A&M | 22.64 | Clemson Opener | 
| 2 | RIVERS, Kasahn |  Thomas (Ga.) | 22.40 | Embry-Riddle Running Elements Classic - Collegiate and Open | 
| 3 | WILLIAMS, Jabari |  Florida A&M | 22.33 | UCF Black & Gold Invite | 
| 4 | NEMBHARD, Andre |  Thomas (Ga.) | 22.21 | UWG Legends Track Meet | 
| 5 | AMEY, Rashad |  Columbus St. | 22.27 | Emory Spring Break Classic | 
| 6 | JACKSON, Demario |  Albany State | 22.36 | UWG Legends Track Meet | 
| 7 | ADENUGA, Adetoro |  Fort Valley State | 22.61 | Bob Hayes Spring Break Invitational - Collegiate | 
| 8 | FRALISH, Ethan |  Thomas (Ga.) | 22.75 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | LUMPRIS, Ethan |  Morehouse | 22.98 | |
| 2 | BOWLES, Victor |  Morehouse | 22.87 | |
| 3 | MCCANTS, Jaquan |  Edward Waters | 22.85 | USC Open Outdoor 2023 | 
| 4 | WHITE, Tracy |  Edward Waters | 22.80 | UCF Knights Invite | 
| 5 | BROOKS, Kenneth |  Columbus St. | 22.80 | |
| 6 | THOMAS, Tyler |  Columbus St. | 22.85 | UWG Legends Track Meet | 
| 7 | ALFORD, Micah | Unattached | 22.92 | |
| 8 | HAYES, Jabari |  Morehouse | 23.06 | 2024 Morehouse Relays | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BROWN JR., Anthony |  Columbus St. | 23.48 | Emory Spring Break Classic | 
| 2 | PRICHARD, Amonyay |  Flagler | 23.32 | Embry-Riddle Running Elements Classic - Collegiate and Open | 
| 3 | HERRON, Daniel | Takeoff track club | 23.15 | |
| 4 | RUDOLPH, Jaden |  Fort Valley State | 23.09 | UWG Legends Invitational | 
| 5 | SHIRLEY, Harold | Unattached | 23.10 | |
| 6 | HARMON, Kade |  Columbus St. | 23.25 | Emory Thrills in the Hills 2024 | 
| 7 | FLORES, Elijah |  Morehouse | 23.36 | Emory Thrills in the Hills 2024 | 
| 8 | MCFARLANE, Christian |  Flagler | 23.49 | Embry-Riddle Running Elements Classic - Collegiate and Open | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PARKER, Kendall |  Columbus St. | NT | |
| 2 | BRYANT, Taquarrion |  Fort Valley State | 24.75 | Clark Atlanta University Panther Track and Field Classic | 
| 3 | ALPHONSE, Evan |  Morehouse | 23.79 | Morehouse Maroon Tiger Invitational | 
| 4 | BROWN, Keashawn | Unattached | 23.50 | |
| 5 | DILLON, Jahmai |  Columbus St. | 23.58 | UWG Legends Invitational | 
| 6 | WATERS, Ivan | UNAT-Morehouse | 24.43 | |
| 7 | HARVEY, Terrance | Indiana | 28.02 | |
| 8 | EBERHART, TaQuarus |  Columbus St. | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | TERRELL, J.T. | Unattached | NT | |
| 3 | GAVITSE, Hernic |  Columbus St. | NT | |
| 4 | STONE, Donovan |  Columbus St. | NT | |
| 5 | VESSAT, Samuel |  Edward Waters | NT |