| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | JACKSON, Lathum | UNAT-Morehouse | 56.88 | |
| 2 | KORTA, Brogan |  Morehouse | 55.47 | |
| 3 | SANFORD, Jaydon |  Florida A&M | 54.32 | UNF East Coast Relays | 
| 4 | SMITH, Jordan |  Albany State | 52.00 | |
| 5 | WALLS, Dante |  Columbus St. | 53.57 | |
| 6 | GRIMES, Gary |  Columbus St. | 55.39 | UWG Legends Invitational | 
| 7 | RAVENSCRAFT, Mark |  Columbus St. | 55.83 | UWG Legends Track Meet | 
| 8 | BAH, Gandor | SWGTC | 57.00 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CALVIN, Anthonyo |  Edward Waters | 1:00.32 | South Florida Invitational | 
| 2 | BELIZAIRE, Reuel |  Flagler | 59.67 | |
| 3 | STANLEY, David |  Flagler | 58.57 | |
| 4 | FOWLER III, Cavitt |  Morehouse | 58.00 | |
| 5 | MILNER, Andre |  Fort Valley State | 58.00 | Bob Hayes Spring Break Invitational - Collegiate | 
| 6 | PASLEY, Reece |  Columbus St. | 59.11 | Clark Atlanta University Panther Track and Field Classic | 
| 7 | JIMENEZ, Kruz |  Flagler | 1:00.12 | |
| 8 | BRUNNER, Matthew |  West Georgia | 1:00.40 | Don McGarey Invitational | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | BROWN, Raheam |  Fort Valley State | 1:10.68 | Clark Atlanta University Panther Track and Field Classic | 
| 3 | PARKER, Caleb |  Columbus St. | 1:04.64 | UWG Legends Track Meet | 
| 4 | HALL, Conner |  Flagler | 1:00.70 | |
| 5 | FLOWERS, Ryan |  Columbus St. | 1:02.00 | UWG Legends Track Meet | 
| 6 | DOLTON, Aaron |  Flagler | 1:04.86 |