| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JACKSON, Lathum | UNAT-Morehouse | 56.88 | |
| 2 | KORTA, Brogan | Morehouse |
55.47 | |
| 3 | SANFORD, Jaydon | Florida A&M |
54.32 | UNF East Coast Relays |
| 4 | SMITH, Jordan | Albany State |
52.00 | |
| 5 | WALLS, Dante | Columbus St. |
53.57 | |
| 6 | GRIMES, Gary | Columbus St. |
55.39 | UWG Legends Invitational |
| 7 | RAVENSCRAFT, Mark | Columbus St. |
55.83 | UWG Legends Track Meet |
| 8 | BAH, Gandor | SWGTC | 57.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CALVIN, Anthonyo | Edward Waters |
1:00.32 | South Florida Invitational |
| 2 | BELIZAIRE, Reuel | Flagler |
59.67 | |
| 3 | STANLEY, David | Flagler |
58.57 | |
| 4 | FOWLER III, Cavitt | Morehouse |
58.00 | |
| 5 | MILNER, Andre | Fort Valley State |
58.00 | Bob Hayes Spring Break Invitational - Collegiate |
| 6 | PASLEY, Reece | Columbus St. |
59.11 | Clark Atlanta University Panther Track and Field Classic |
| 7 | JIMENEZ, Kruz | Flagler |
1:00.12 | |
| 8 | BRUNNER, Matthew | West Georgia |
1:00.40 | Don McGarey Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BROWN, Raheam | Fort Valley State |
1:10.68 | Clark Atlanta University Panther Track and Field Classic |
| 3 | PARKER, Caleb | Columbus St. |
1:04.64 | UWG Legends Track Meet |
| 4 | HALL, Conner | Flagler |
1:00.70 | |
| 5 | FLOWERS, Ryan | Columbus St. |
1:02.00 | UWG Legends Track Meet |
| 6 | DOLTON, Aaron | Flagler |
1:04.86 |