| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | DOLL, Alexis |  West Georgia | 15.41 | UWG Legends Track Meet | 
| 2 | RENTERIA-SILVA, Valeyna |  West Georgia | 15.14 | UWG Legends Track Meet | 
| 3 | THOMPSON, Mayah |  Albany State | 15.11 | 2024 Morehouse Relays | 
| 4 | MADISON, Briana |  Fort Valley State | 14.85 | SIAC Championships | 
| 5 | ALBERT, DeAna |  Albany State | 15.00 | |
| 6 | WALKER, Kennedy |  Albany State | 15.12 | 2024 Morehouse Relays | 
| 7 | FULFORD, Olivia |  West Georgia | 15.25 | UWG Legends Track Meet | 
| 8 | UWAOMAH, Chioma |  West Georgia | 15.48 | 2023 Gulf South Conference Championship | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BAILEY, Tyloni |  Albany State | 17.50 | UWG Legends Track Meet | 
| 2 | VARNUM, Liza |  Columbus St. | 16.82 | Emory Thrills in the Hills 2024 | 
| 3 | KLECKLEY, Kaleah |  Albany State | 16.16 | Emory Spring Break Classic | 
| 4 | HARRIS, Janiya |  Albany State | 15.90 | Emory Spring Break Classic | 
| 5 | KERN, Julianna |  Albany State | 15.98 | Emory Spring Break Classic | 
| 6 | OSBORNE, Elizabeth |  West Georgia | 16.67 | UWG Legends Track Meet | 
| 7 | TUCKER, Tytiyana |  Fort Valley State | 16.90 | 2024 Morehouse Relays | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | ZINNIE, Zoe |  Flagler | NT | |
| 4 | LACY, Syni |  Columbus St. | 17.79 | UWG Legends Track Meet | 
| 5 | ROBINSON, Selena |  Florida A&M | NT |