| * | Rob and Patty Lydic Meet Record | 1:02.67 | Maley, Mariah | St. Francis (Ind.) | 1/17/2025 | Ohio Northern-King Horn Sports Center |
| % | SportsCenter Record | 55.46 | Amber James | Wheaton (Mass.) | 2002 | |
| $ | ONU School Record | 57.38 | Emily Richards | Ohio Northern | 2016 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | COCHRAN, Miniyah | UNSF | 1:05.22 | |
| 3 | BRUMBAUGH, Lillian | FR | St. Francis (Ind.) |
1:05.20 |
| 4 | TEMPLE, Vanessa | SR | Indiana East |
1:03.89 |
| 5 | WALSH, Tatum | SO | Ohio Northern |
1:00.46 |
| 6 | ROHDE, Mackenzie | SO | Indiana East |
1:00.48 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 4 | GURNEY, Madison | FR | St. Francis (Ind.) |
1:14.70 |
| 5 | DALE, Abigale | UNSF | 1:07.00 | |
| 6 | JACKSON, Jillian | SO | Indiana East |
1:08.43 |