| * | Larry Cole Meet Record | 26.05 | Asia Gardner | Siena Heights | 2020 |
| % | SportsCenter Record | 25.01 | Mary Mahoney | Mt. Union | 2013 |
| $ | ONU School Record | 25.96 | Taylor Lavey | Ohio Northern | 2018 |
| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | FATIGATI, Jessica | Westminster (Pa.) |
26.40 |
| 2 | DEBORD, Paris | Rio Grande |
26.63 |
| 3 | SCHWELLER, Claire | Ohio Northern |
26.99 |
| 4 | RUDOLPH, Emma | Westminster (Pa.) |
27.21 |
| 5 | GROOMS, Kendra | Rio Grande |
27.21 |
| 6 | PAINTER, Abigail | Westminster (Pa.) |
27.80 |
| 7 | SALAZAR, Isabelle | Adrian |
27.81 |
| 8 | ZENDRON, Jenna | Westminster (Pa.) |
27.91 |
| 9 | MATHEWS, Amanda | Adrian |
28.17 |
| 10 | GOODRICH, Caitlyn | Westminster (Pa.) |
28.60 |
| 11 | MYERS, Kinsey | Bluffton |
28.70 |
| 12 | BEAUDOIN, Cailey | Adrian |
28.71 |
| 13 | HESLET, Ciara | Adrian |
29.14 |
| 14 | WEININGER, Lindsay | Ohio Northern |
29.32 |
| 15 | PETERSON, Emily | Adrian |
29.35 |
| 16 | ERNSTES, Hilary | Manchester |
30.00 |
| 17 | WOLTERS, Lauryn | Ohio Northern |
30.10 |
| 18 | TREHARN, Missy | Westminster (Pa.) |
30.75 |
| 19 | BOARDMAN, Jaclyn | Ohio Northern |
31.13 |
| 20 | JEFFCOAT, Alyssa | Westminster (Pa.) |
32.40 |
| 21 | BOLYARD, Madison | Ohio Northern |
33.45 |
| 22 | KERR, Kailea | Bluffton |
NT |
| 23 | HYDRE-YUSOFF, Jameela | Manchester |
NT |
| 24 | DUNCAN, Emily | Rio Grande |
NT |