| * | Larry Cole Meet Record | 3:30.44 | Ohio Northern | 1/21/2022 | Ohio Northern-King Horn Sports Center | |
| % | SportsCenter Record | 3:15.22 | Rowan | 1999 | ||
| $ | ONU School Record | 3:17.15 | Amstutz, Huntley, Heyman, O'Brien | Ohio Northern | 2009 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Bluffton |
A | McCoy | Gist | Lough | Shawk | 3:26.00 |
| 2 | Ohio Northern |
A | O'Neill | Zimmerer | Conn | Gross | 3:27.00 |
| 3 | Adrian |
A | Clemens | Wright | Cousino | Kvalevog | 3:28.00 |
| 4 | Rio Grande |
A | Adams | Beverly | Larue | Whitley | 3:30.87 |
| 5 | Westminster (Pa.) |
A | Kamenski | Beard | Jones | White | 3:32.00 |
| 6 | Adrian |
B | Trudell | Moore | Vines | Ewing | 3:33.00 |
| 7 | Ohio Northern |
B | Byers | Wakefield | Nichols | Vance | 3:33.00 |
| 8 | Westminster (Pa.) |
B | Alexander | White | Mull | Pratt | 3:39.00 |
| 9 | Bluffton |
B | Ly | Roudebush | Brien | Dodson | 3:45.00 |
| 10 | Westminster (Pa.) |
C | Crow | Koehler | Campbell | Bailor | 3:48.00 |