| * | Larry Cole Meet Record | 4:08.19 | Hernandez, Short, Harris, Stersic | Ohio Northern | 2020 |
| % | SportsCenter Record | 3:48.78 | Wheaton (Mass.) | 2002 | |
| $ | ONU School Record | 3:55.67 | Fisher, Lavey, Ewing, Richards | Ohio Northern | 2018 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Manchester |
A | Papazoglou | Faulkner | Chupp | Ferris | NT |
| 2 | Adrian |
B | Sempf | Shiels | Brown | Smith | 4:45.00 |
| 3 | Bluffton |
A | Myers | Kerr | Rider | Schifer | DNF |
| 4 | Adrian |
A | Mougrabi | Dunscombe | Raftis | Vea | 4:35.00 |
| 5 | Cedarville |
A | Ansiel | Atkins | Peramaki | Brown | 4:14.11 |
| 6 | Cedarville |
B | Mulder | Hartenburg | Fuller | Lichtenberger | DNS |